5.結(jié)論

首先，表面張力測量證明牛精漿具有很好的表面活性，可能與BSP蛋白有關(guān)。其次，Langmuir膜法（先前被證明是公牛精子外細(xì)胞膜的相關(guān)脂質(zhì)單層模型[35]）有助于表征BSP蛋白對脂質(zhì)膜的親和力。BSP蛋白能夠到達(dá)被磷脂覆蓋的牛精子細(xì)胞表面，這得益于它們與磷脂酰膽堿的相互作用和自身的表面活性。我們的假設(shè)是，由于它們自身的表面活性，它們首先穿透精子的外小葉，然后由于與磷脂酰膽堿的強(qiáng)烈相互作用而留在那里。最后，利用Langmuir膜法對已知的BSP蛋白螯合劑如ldl和脂質(zhì)體的作用進(jìn)行了篩選。脂質(zhì)體與低密度脂蛋白一樣有效地阻止BSP蛋白插入磷脂層。低密度脂蛋白和脂質(zhì)體的比例相同：0.16–0.17 mg磷脂酰膽堿/mg BSP。我們還不知道在冷凍保存的時間尺度上脂質(zhì)體的非常微弱的插入是否有一些生物學(xué)后果。需要進(jìn)一步研究分析每種BSP蛋白的表面性質(zhì)及其在膜上的作用。此外，可以測試Langmuir膜，以檢測BSP與膜的親和力隨溫度的變化[20]。最后，Langmuir膜法是一種有效的篩選BSP蛋白質(zhì)新螯合劑的方法。該方法可適用于其他動物物種。

致謝

作者感謝IMV Technologies（法國）的財務(wù)支持，Gérard Chatagnon負(fù)責(zé)精漿蛋白質(zhì)定量，Véronique Solé負(fù)責(zé)凝膠電泳，Maureen Collobert負(fù)責(zé)英語校正，Alain Sire和Patrice Papineau負(fù)責(zé)手套箱的構(gòu)思和實現(xiàn)，允許在受控大氣中使用朗繆爾槽。

附錄A.補(bǔ)充數(shù)據(jù)

與本文相關(guān)的補(bǔ)充數(shù)據(jù)可在在線版本中找到，網(wǎng)址為http://dx.doi.org/10.1016/j.colsurfb.2015.11.34

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