四、結論

在這項工作中，我們研究了 16-E2-16 與 通過使用紫外-可見光、熒光和 CD 光譜技術結合計算方法來計算 Hb。 此外， 該相互作用系統也采用表面張力法。 在 UV-vis 研究的幫助下，我們發現 16-E2-16 導致血紅素基團暴露于水性介質。 熒光光譜證實內在猝滅 16-E2-16 的滲透抑制了血紅素對 Trp 的影響 進入 Hb 的疏水腔并導致熒光強度增加。 效果還是比較明顯的 只到cmc。 Trp 熒光比 Tyr 受到的影響更大 如同步光譜所示。 表面張力結果 確定 16-E2-16 的 cmc 值在存在的情況下增加 Hb 以及隨著溫度升高而 cac 保持不變 在所有溫度下保持恒定。 表面張力測量也 表明膠束化過程是自發的、放熱的和 熵驅動。 此外，得出結論，吸附在 界面優于膠束化。 分子建模方法表明 16-E2-16 在 Hb 中的結合 位于α1和β2鏈中。 它還提供了對我們的驗證 通過確認主要結合力的實驗結果 是疏水的和靜電的。 圓二色性表明 16-E2-16 與 Hb 結合導致 α-螺旋含量減少。 MD 模擬結果表明 Hb 和 16-E2-16 結合 Hb 穩定在 6000 ps 左右并確認構象 與 16-E2-16 相互作用后 Hb 發生了變化，如 我們的 CD 結果顯示。

致謝

Rajan Patel 博士非常感謝來自 科學與工程研究委員會（SR/S1/PC-19/2011 和 SB/EMEQ-097/2013) 和新德里大學撥款委員會， 印度（F.No. 39-841/2010 (SR)）。 Abbul Bashar Khan 博士表示感謝 印度新德里科學與工程研究委員會， 提供研究資助 (SB/FT/CS-031/2013)。 作者還 感謝 DST 為 FIST 撥款提供制裁令編號。 （SR/FIST/LS-541/2012）。

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酯功能化的雙子表面活性劑與血紅蛋白的結合——摘要、簡介

酯功能化的雙子表面活性劑與血紅蛋白的結合——材料和方法

酯功能化的雙子表面活性劑與血紅蛋白的結合——結果和討論

酯功能化的雙子表面活性劑與血紅蛋白的結合——結論、致謝！